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Image Title: DNA fragmentation characteristic of programmed cell death (PCD) detected in NaCl-treated determinate primary roots of Stenocereus gummosus (Cactaceae)
Image Credit: Svetlana Shishkova, Universidad Nacional Autónoma de México, Joseph G. Dubrovsky, Universidad Nacional Autónoma de México
Intended End User: Teacher, Student
License Details: BSA - Terms for Image Use
Copyright held by: Svetlana Shishkova, Joseph G. Dubrovsky, BSA
Date Created: 9/1/2005
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About the Image

DNA fragmentation characteristic of programmed cell death (PCD) was detected in NaCl-treated determinate primary roots of Stenocereus gummosus (Cactaceae) using the terminal deoxynucleotide transferase-mediated dUTP nick-end labeling (TUNEL) assay. Nuclei in the bottom left image, a longitudinal section of a root tip after PCD-inducing NaCl treatment, fluoresce blue after 4'-6-diamidino-2-phenylindole (DAPI) staining. Those nuclei undergoing DNA fragmentation fluoresce green in the same section (top left, fluorescein-isothiocyanate [FITC] staining). During root development, PCD was not involved in meristem exhaustion, but did occur in root-hair and root-cap cells. Both DNA and nuclear fragmentation were detected in a root-hair cell (right: top, FITC; middle, DAPI). Dead remnants (red-fluorescing nuclei, propidium-iodine stain, bottom right) of the root cap lie below a root that has terminated growth.

Link to the AJB Abstract for the article:

Developmental programmed cell death in primary roots of Sonoran Desert Cactaceae

National Science Foundation  Development Supported by the National Science Foundation