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American Journal of Botany
AJB Primer Notes & Protocols in the Plant Sciences

For AJB Primer Notes & Protocols in the Plant Sciences Instructions for Authors, go to http://www.botany.org/ajb/PNP_Online_instructions.html.

AJB Primer Notes & Protocols in the Plant Sciences is an online-only section of the American Journal of Botany (AJB) intended to promote rapid dissemination of protocols used in genetic analyses of plants. Subjects appropriate for this section include: (1) marker notes providing primer sequences for microsatellite or other markers in particular taxa, and (2) protocol notes describing new methods for isolating, visualizing, or scoring genetic markers. Protocol notes are expected to provide evidence that the new protocol increases the accuracy or reproducibility of marker scoring, decreases the time or expense of marker scoring, or provides the ability to score new classes of markers.

For primer notes, the intent is to demonstrate the usefulness of newly developed primers for subsequent, more detailed studies.  Sampling distribution and numbers will depend on the life history characteristics of the organism under study, but sample sizes should be adequate to support the genetic parameters presented.  For example, sample sizes are expected to be greater for abundant and widely distributed species than for rare species.  Whenever possible, the use of data from multiple populations is encouraged to capture a wider range of potential variation.  Although most primers are expected to exhibit polymorphism, monomorphic primers are also acceptable because they may be useful in other populations or related taxa.  Given the limited sampling and biological characteristics of some species (small population sizes, recent colonization, admixture, clonality, etc.), tests of linkage disequilibrium and Hardy-Weinberg equilibrium are not required for primer notes articles but are instead reserved for later in-depth population surveys.


 
From American Journal of Botany 98(10): 2011
Racquel A. Kriedt, Aline M. C. Ramos-Fregonezi, Luciano B. Beheregaray, Sandro L. Bonatto, and Loreta B. Freitas

Am. J. Bot. 2011 98: e277-e279. Published online 22 September, 2011; doi:10.3732/ajb.1100178 [Abstract] [Full Text] [PDF] 

Eric S. J. Harris and Matthew R. Klooster

Am. J. Bot. 2011 98: e293-e295. Published online 22 September, 2011; doi:10.3732/ajb.1100190 [Abstract] [Full Text] [PDF] 

Mario Vallejo-Marín, Lislie Solis-Montero, Cecile F. E. Bacles, and Olivier Lepais

Am. J. Bot. 2011 98: e296-e299. Published online October 2011; doi:10.3732/ajb.1100159 [Abstract] [Full Text] [PDF] 

Yuppayao Kophimai, Carolina Cornejo, Markus Peintinger, Ariel Bergamini, and Christoph Schidegger

Am. J. Bot. 2011 98: e290-e292. Published online 16 September, 2011; doi:10.3732/ajb.1100144 [Abstract] [Full Text] [PDF] 

A. Jesús Muñoz-Pajares, M. Belén Herrador, Mohamed Abdelaziz, F. Xavier Picó, Timothy F. Sharbel, José M. Gómez, and Francisco Perfectti

Am. J. Bot. 2011 98: e287-e289. Published online 22 September, 2011; doi:10.3732/ajb.1100181 [Abstract] [Full Text] [PDF] 

Jinghu Jiang, Jing Wang, Ming Kang, Wei Sun, and Hongwen Huangand

Am. J. Bot. 2011 98: e284-e286. Published online 22 September, 2011; doi:10.3732/ajb.1100221 [Abstract] [Full Text] [PDF] 

Glyn M. Figueira, Miklos Maximiliano Bajay, Carolina M. S. Silva, Maria I. Zucchi, Mariza Monteiro, and Marili V. N. Rodrigues

Am. J. Bot. 2011 98: e282-e283. Published online October 2011; doi:10.3732/ajb.1100221 [Abstract] [Full Text] [PDF] 

Ari Novy and Kenneth C. Jones

Am. J. Bot. 2011 98: e280-e281. Published online 16 September 2011; doi:10.3732/ajb.1100196 [Abstract] [Full Text] [PDF] 

 
From American Journal of Botany 98(5): 2010
Lee Hong Tnah, Chai Ting Lee, Soon Leong Lee, Kevin Kit Siong Ng, Chin Hong Ng, and Siaw San Hwang

Am. J. Bot. 2011 98: e130-e132. Published online May 2, 2011; doi:10.3732/ajb.1000469 [Abstract] [Full Text] [PDF] 

Inga M. Michalczyk, Claudia Schumacher, Christina Mengel, Ilona Leyer, and Sascha Liepelt
Identification and characterization of 12 microsatellite loci in Cnidium dubium (Apiaceae) using next-generation sequencing
Am. J. Bot. 2011 98: e127-e129. Published online May 2, 2011; doi:10.3732/ajb.1000429 [Abstract] [Full Text] [PDF] 

Ai-Hong Yang, Jin-Ju Zhang, Xiao-Hong Yao, and Hong-Wen Huang

Am. J. Bot. 2011 98: e123-e126. Published online May 11, 2011; doi:10.3732/ajb.1000532 [Abstract] [Full Text] [PDF] 

Chloé E. L. Delmas, Emeline Lhuillier, André Pornon, and Nathalie Escaravage

Am. J. Bot. 2011 98: e120-e122. Published online May 11, 2011; doi:10.3732/ajb.1000533 [Abstract] [Full Text] [PDF] 

Cheng Choon Ang, Soon Leong Lee, Chai Ting Lee, Lee Hong Tnah, Rozainah Mohamad Zakaria, and Ching Ching Ng

Am. J. Bot. 2011 98: e117-e119. Published online May 2, 2011; doi:10.3732/ajb.1000494 [Abstract] [Full Text] [PDF] 

Uploaded January 21, 2011 AJB Advance Access
Zhi-Hua Wu, Shu-Zhen Wang, Ji-Hong Hu, Feng Li, Wei-Dong Ke, and Yi Ding

Am. J. Bot. published online January 21, 2011, doi:10.3732/ajb.1000434 [Abstract] [PDF] 

En-Wei Tian, Hui Yu, Da-Yong Zhang, and John D. Nason

Am. J. Bot. published online January 21, 2011, doi:10.3732/ajb.1000432 [Abstract] [PDF] 

Sylvie V. M. Tesson, Marco Borra, Wiebe H. C. F. Kooistra, and Gabriele Procaccini

Am. J. Bot. published online January 21, 2011, doi:10.3732/ajb.1000430 [Abstract] [PDF] 

Susanne Schreiter, Susan K. Ebeling, and Walter Durka

Am. J. Bot. published online January 21, 2011, doi:10.3732/ajb.1000417 [Abstract] [PDF] 
Uploaded January 5, 2011 AJB Advance Access
Mariela C. Núñez-Ávila, María Uriarte, Pablo A. Marquet, and Juan J. Armesto

Am. J. Bot. published online January 5, 2011, doi:10.3732/ajb.1000425 [Abstract] [PDF] 

Yu Ma, Tao Yang, Jianping Guan, Shumin Wang, Haifei Wang, Xuelian Sun, and Xuxiao Zong

Am. J. Bot. published online January 5, 2011, doi:10.3732/ajb.1000407 [Abstract] [PDF] 

Kelsey L. Glennon and Sheri A. Church

Am. J. Bot. published online January 5, 2011, doi:10.3732/ajb.1000294 [Abstract] [PDF] 
Uploaded December 30, 2010 AJB Advance Access
Annika M. Moe and George D. Weiblen

Am. J. Bot. published online December 30, 2010, doi:10.3732/ajb.1000412 [Abstract] [PDF] 

Lin Li, Fang-Yun Cheng, and Qi-Xiang Zhang

Am. J. Bot. published online December 30, 2010, doi:10.3732/ajb.1000410 [Abstract] [PDF] 
From American Journal of Botany 97(12): 2010
Fangming Zhang, Tao Su, Ying Yang, Yanhong Zhai, Yunheng Ji, and Shaotian Chen

Am. J. Bot. 2010 97: e159-e161. Published online November 15, 2010; doi:10.3732/ajb.1000377 [Abstract][Full Text] [PDF] 

Ye Sun, Yi-Fei Liu, Jing Wang, Yong Guo, and Hong-Wen Huang

Am. J. Bot. 2010 97: e157-e158. Published online November 15, 2010; doi:10.3732/ajb.1000332 [Abstract][Full Text] [PDF] 

Jian-Qiang Ma, Yan-Hua Zhou, Chun-Lei Ma, Ming-Zhe Yao, Ji-Qiang Jin, Xin-Chao Wang, and Liang Chen

Am. J. Bot. 2010 97: e153-e156. Published online November 5, 2010; doi:10.3732/ajb.1000376 [Abstract][Full Text] [PDF] 

Kenneth C. Oberlander, Léanne L. Dreyer, and Francois Roets

Am. J. Bot. published online November 5, 2010, doi:10.3732/ajb.1000390 [Abstract] [PDF] 

Jian-Qiang Ma, Yan-Hua Zhou, Chun-Lei Ma, Ming-Zhe Yao, Ji-Qiang Jin, Xin-Chao Wang, and Liang Chen

Am. J. Bot. published online November 5, 2010, doi:10.3732/ajb.1000376 [Abstract] [PDF] 

Péter Poczai, István Cernák, Ahmad Mosapour Gorji, Sándor Nagy, János Taller, and Zsolt Polgár

Am. J. Bot. published online November 5, 2010, doi:10.3732/ajb.1000360 [Abstract] [PDF] 

Hong-Wei Wang, Bing Zhang, Zhao-Shan Wang, Yue-Qin Cheng, and Yong-Zhong Ye

Am. J. Bot. published online October 27, 2010, doi:10.3732/ajb.1000334 [Abstract] [PDF] 

Ricardo Riegel, Leila Diaz, and Débora Véliz

Am. J. Bot. published online October 27, 2010, doi:10.3732/ajb.1000314 [Abstract] [PDF] 
 
Uploaded October 25, 2010 AJB Advance Access
Stéphanie Robert, Adrien Rieux, Xavier Argout, Jean Carlier, and Marie-Françoise Zapater

Am. J. Bot. published online October 25, 2010, doi:10.3732/ajb.1000295 [Abstract] [PDF]
From American Journal of Botany 97(11): 2010
Frédérique Ollitrault, Javier Terol, Jose Antonio Pina, Luis Navarro, Manuel Talon, and Patrick Ollitrault

Am. J. Bot. 2010 97: e124-e129. Published online October 14, 2010; doi:10.3732/ajb.1000280 [Abstract] [Full Text] [PDF] 

Juan Viruel, Pilar Catalán, and José Gabriel Segarra-Moragues

Am. J. Bot. 2010 97: e121-e123. Published online October 8, 2010; doi:10.3732/ajb.1000304 [Abstract] [Full Text] [PDF] 

Zhao-Shan Wang, Hai-Qin Sun, Hong-Wei Wang, and Song Ge

Am. J. Bot. 2010 97: e117-e120. Published online October 8, 2010; doi:10.3732/ajb.1000270 [Abstract] [Full Text] [PDF] 

Wei Wu, Ren-Chao Zhou, Hui-Run Huang, and Xue-Jun Ge

Am. J. Bot. 2010 97: e114-e116. Published online September 29, 2010; doi:10.3732/ajb.1000327 [Abstract] [Full Text] [PDF] 

G. J. Sexton, C. H. Frere, M. J. Dieters, I. D. Godwin, and P. J. Prentis

Am. J. Bot. 2010 97: e111-e113. Published online September 27, 2010; doi:10.3732/ajb.1000300 [Abstract] [Full Text] [PDF] 

F. W. Cidade, T. T. de Souza-Chies, F. H. D. Souza, L. A. R. Batista, M. Dall’Agnol, J. F. M. Valls, M. I. Zucchi, and A. P. Souza

Am. J. Bot. 2010 97: e107-e110. Published online October 1, 2010; doi:10.3732/ajb.1000207 [Abstract] [Full Text] [PDF] 

Juanita Choo, Heather Ishak, Beryl Simpson, Ulrich Mueller, and Tom Juenger

Am. J. Bot. 2010 97: e105-e106. Published online October 1, 2010; doi:10.3732/ajb.1000281 [Abstract] [Full Text] [PDF]
From American Journal of Botany 97(10): 2010
Nicolas Magain, Laura L. Forrest, Emmanuël Sérusiaux, and Bernard Goffinet

Am. J. Bot. 2010 97: e102-e104. Published online September 22, 2010; doi:10.3732/ajb.1000283 [Abstract] [Full Text] [PDF] 

Margaret C. Jewell, Celine H. Frere, Peter J. Prentis, Christopher J. Lambrides, and Ian D. Godwin

Am. J. Bot. 2010 97: e99-e101. Published online September 10, 2010; doi:10.3732/ajb.1000254 [Abstract] [Full Text] [PDF] 

Brendan L. Jones, Michelle Waycott, Heather L. A. Robson, Ainsley Calladine, and Tony Page

Am. J. Bot. 2010 97: e97-e98. Published online September 16, 2010; doi:10.3732/ajb.1000213 [Abstract] [Full Text] [PDF] 

Liyan Zeng, Zhonghu Li, Tsering Tashi, Jian Chen, Yang Zhong, and Yupeng Geng

Am. J. Bot. 2010 97: e94-e96. Published online September 10, 2010; doi:10.3732/ajb.1000260 [Abstract] [Full Text] [PDF] 

Lu Zhang and Qiao-Ming Li

Am. J. Bot. 2010 97: e91-e93. Published online September 10, 2010; doi:10.3732/ajb.1000245 [Abstract] [Full Text] [PDF] 

Ji-Qing Wei, Zong-You Chen, Zheng-Feng Wang, Hui Tang, Yun-Sheng Jiang, Xiao Wei, Xiao-Yi Li, and Xiao-Xue Qi
Isolation and characterization of polymorphic microsatellite loci in Camellia nitidissima Chi (Theaceae)
Am. J. Bot. 2010 97: e89-e90. Published online September 10, 2010; doi:10.3732/ajb.1000234 [Abstract] [Full Text] [PDF] 
Nabeeh A. Hasan, Klaus Mummenhoff, Carlos F. Quiros, C. David Tay, and C. Donovan Bailey

Am. J. Bot. 2010 97: e85-e88. Published online September 16, 2010; doi:10.3732/ajb.1000225 [Abstract] [Full Text] [PDF]
From American Journal of Botany 97(9): 2010
Gustavo M. Mori, Maria I. Zucchi, Iracilda Sampaio, and Anete P. Souza

Am. J. Bot. published online August 6, 2010, doi:10.3732/ajb.1000219 [Abstract] [Full Text] [PDF] 
Chen Chen, Yi-Hui Liu, Cheng-Xing Fu, and Ying-Xiong Qiu

Am. J. Bot. published online August 6, 2010, doi:10.3732/ajb.1000165 [Abstract] [Full Text] [PDF]
From American Journal of Botany 97(8): 2010
Shu-Zhen Wang, Lei Pan, Kan Hu, Chan-You Chen, and Yi Ding

Am. J. Bot. 2010 97: e75-e78. Published online July 26, 2010; doi:10.3732/ajb.1000153 [Abstract] [Full Text] [PDF] 
Lorena B. Parra Gonzalez, Shannon C. K. Straub, Jeff J. Doyle, Paula E. Mora Ortega, Haroldo E. Salvo Garrido, and Iván J. Maureira Butler
Development of microsatellite markers in Lupinus luteus (Fabaceae) and cross-species amplification in other lupine species
Am. J. Bot. 2010 97: e72-e74. Published online July 26, 2010; doi:10.3732/ajb.1000170 [Abstract] [Full Text] [PDF]
From American Journal of Botany 97(7): 2010
Ya-Ming Gong, Sheng-Chun Xu, Wei-Hua Mao, Qi-Zan Hu, Gu-Wen Zhang, Ju Ding, and Ze-Yun Li

Am. J. Bot. 2010 97: e69-e71. Published online June 22, 2010; doi:10.3732/ajb.1000166 [Abstract] [Full Text] [PDF] 

Dirk U. Bellstedt, Michael D. Pirie, J. Christiaan Visser, Margaret J. de Villiers, and Berit Gehrke

Am. J. Bot. 2010 97: e65-e68. Published online July 1, 2010; doi:10.3732/ajb.1000181 [Abstract] [Full Text] [PDF] 

Glyn M. Figueira, Pamela R. Ramelo, Daniela C. Ogasawara, Ilio Montanari, Jr., Maria I. Zucchi, Marcelo M.Cavallari, and Mary A. Foglio

Am. J. Bot. 2010 97: e63-e64. Published online June 22, 2010; doi:10.3732/ajb.1000145 [Abstract] [Full Text] [PDF] 
Xiao-Yi Li, Zheng-Feng Wang, Peng Zhu, Wan-Hui Ye, Xiao-Feng Huang, Lin-Fang Wu, Sheng-Feng Chai, and Hong-Lin Cao

Am. J. Bot. 2010 97: e61-e62. Published online June 22, 2010; doi:10.3732/ajb.1000136 [Abstract] [Full Text] [PDF] 
Sergio Currò, Marco Caruso, Gaetano Distefano, Alessandra Gentile, and Stefano La Malfa

Am. J. Bot. 2010 97: e58-e60. Published online June 16, 2010; doi:10.3732/ajb.1000143 [Abstract] [Full Text] [PDF]
From American Journal of Botany 97(6): 2010
Na-Na Xu, Shuo Yu, Jun-guo Zhang, Po Keung Eric Tsang, and Xiao-Yong Chen

Am. J. Bot. 2010 97: e56-e57; doi:10.3732/ajb.1000111 [Abstract] [Full Text] [PDF] 
Kurt M. Neubig and J. Richard Abbott

Am. J. Bot. 2010 97: e52-e55; doi:10.3732/ajb.1000128 [Abstract] [Full Text] [PDF] 

Yi Sun, Cheng-Xing Fu, and Ying-Xiong Qiu
Development, characterization, and transferability of microsatellite markers for Kirengeshoma palmata (Hydrangeaceae)
Am. J. Bot. 2010 97: e48-e51; doi:10.3732/ajb.1000134 [Abstract] [Full Text] [PDF] 
Serena Terzoli, Isacco Beritognolo, Maurizio Sabatti, and Elena Kuzminsky

Am. J. Bot. 2010 97: e45-e47. Published online May 21, 2010; doi:10.3732/ajb.1000112 [Abstract] [Full Text] [PDF] 

Andreas Homolka, Maria Berenyi, Kornel Burg, Dieter Kopecky, and Silvia Fluch

Am. J. Bot. 2010 97: e42-e44. Published online May 24, 2010; doi:10.3732/ajb.1000127 [Abstract] [Full Text] [PDF] 
Yong Li, Lu Liang, and Xue-Jun Ge

Am. J. Bot. 2010 97: e39-e41. Published online May 7, 2010; doi:10.3732/ajb.1000098 [Abstract] [Full Text] [PDF]
From American Journal of Botany 97(5): 2010
Susan M. Kabat, Christopher W. Dick, and Mark D. Hunter

Am. J. Bot. 2010 97: e37-e38. Published online April 14, 2010; doi:10.3732/ajb.1000064 [Abstract] [Full Text] [PDF] 
Kathrin Luettmann, Inga M. Michalczyk, Christina Mengel, Birgit Ziegenhagen, Eckhard W. Heymann, Pedro P. Pinedo Saboya, and Ronald Bialozyt

Am. J. Bot. 2010 97: e34-e36. Published online April 19, 2010; doi:10.3732/ajb.1000096 [Abstract] [Full Text] [PDF] 
Jana Raabová, Guillaume Hans, Ange-Marie Risterucci, Anne-Laure Jacquemart, and Olivier Raspé

Am. J. Bot. 2010 97: e31-e33. Published online April 14, 2010; doi:10.3732/ajb.1000084 [Abstract] [Full Text] [PDF] 
Juan M. Arroyo, Cristina Rigueiro, Rocío Rodríguez, Arndt Hampe, Alfredo Valido, Francisco Rodríguez-Sánchez, and Pedro Jordano

Am. J. Bot. 2010 97: e26-e30. Published online April 1, 2010; doi:10.3732/ajb.1000069 [Abstract] [Full Text] [PDF] 
Rafael G. Albaladejo, Federico Sebastiani, Santiago C. González-Martínez, Juan P. González-Varo, Giovanni G. Vendramin, and Abelardo Aparicio

Am. J. Bot. 2010 97: e23-e25. Published online March 26, 2010; doi:10.3732/ajb.1000060 [Abstract] [Full Text] [PDF] 
From American Journal of Botany 97(4): 2010
Jessica R. Brzyski

Am. J. Bot. 2010 97: e20-e22. Published online February 25, 2010, doi:10.3732/ajb.1000008 [Abstract] [Full Text] [PDF] 
From American Journal of Botany 97(3): 2010
Mónica Arakaki, Douglas E. Soltis, Pamela S. Soltis, and Pablo R. Speranza

Am. J. Bot. 2010 97: e17-e19. Published online February 19, 2010; doi:10.3732/ajb.1000026 [Abstract] [Full Text] [PDF] 

Yan Liu, Xue-Jun Ge, Qi-Bao Sun, and Tong Cao

Am. J. Bot. 2010 97: e14-e16. Published online February 16, 2010; doi:10.3732/ajb.1000014 [Abstract] [Full Text] [PDF] 

Violeta I. Simón, F. Xavier Picó, and Juan Arroyo

Am. J. Bot. 2010 97: e10-e13. Published online February 19, 2010; doi:10.3732/ajb.1000023 [Abstract] [Full Text] [PDF] 
Theresa M. Culley and J. Ryan Stewart

Am. J. Bot. 2010 97: e7-e9. Published online January 29, 2010; doi:10.3732/ajb.0900394 [Abstract] [Full Text] [PDF] 
 
From American Journal of Botany 97(2): 2010
Sara López-Vinyallonga, Mónica Arakaki, Núria Garcia-Jacas, Alfonso Susanna, Matthew A. Gitzendanner, Douglas E. Soltis, and Pamela S. Soltis

Am. J. Bot. 2010 97: e4-e6. Published online January 15, 2010; doi:10.3732/ajb.0900376 [Abstract] [Full Text] [PDF] 
 
From American Journal of Botany 97(1): 2010
Rachel Prunier and Andrew Latimer

Am. J. Bot. 2010 97: e1-e3. Published online December 11, 2009; doi:10.3732/ajb.0900326 [Abstract] [Full Text] [PDF]

 

Questions? Contact the Editorial Office
American Journal of Botany
Editorial Office
P.O. Box 299
St. Louis, MO 63166-0299
phone: 314-577-5112; fax: 314-577-9515
E-mail: ajb{at}botany.org

INSTRUCTIONS FOR AUTHORS (in PDF)


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